FastQCFastQC Report
Tue 17 Sep 2019
HFJFLBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJFLBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28544411
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2755980.965505996953309No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC301850.053.54483813
GCACACG314500.052.98066311
CGTCTGA297900.052.6217516
CGGAAGA362950.052.45814
ATCGGAA374850.052.3635372
ACGTCTG300550.052.35587715
TCGGAAG366800.052.2023473
CACGTCT304000.052.19928414
GATCGGA374000.052.1284871
CACACGT316800.052.0666812
AGAGCAC402350.044.2214058
AGCACAC395450.043.6572310
GAACTCC344600.043.6121221
CTGAACT377650.040.95259519
TGAACTC394350.038.93541320
AACTCCA391400.037.78092222
GAAGAGC503300.036.3399776
TCTGAAC433300.036.1537318
CTCCAGT363400.035.98546624
GAGCACA493850.035.7164889
Produced by FastQC (version 0.11.5)