FastQCFastQC Report
Tue 17 Sep 2019
HFJFLBGXC_n01_mb264.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJFLBGXC_n01_mb264.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36797491
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1268890.34483057554114216TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG176850.058.88235546
CGTATGC176450.058.73843844
TCGTATG166050.058.4757243
TGCCGTC177500.056.47513648
GCCGTCT166650.056.413349
ATGCCGT181850.055.81945447
CTCGTAT174100.055.55114742
CATCTCG181650.053.99223339
TCGCGTA15150.053.8335541
ATCTCGT181150.053.46541240
TCTCGTA178700.053.04371341
CGTCTTC176350.052.45667651
CCGTCTT183150.052.38196650
ATCGCGT18750.048.91050340
CGCGTAT17600.048.32863642
GTATGCC227450.046.4142245
CGTCTGA277350.046.2232716
CATCGCG22200.045.40859639
CTTAGGC287400.044.3744433
ACGTCTG292450.043.93243415
Produced by FastQC (version 0.11.5)