FastQCFastQC Report
Tue 17 Sep 2019
HFJFLBGXC_n01_mb263.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJFLBGXC_n01_mb263.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33093244
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC8792552.656901813554452TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG982100.065.3758239
CTCGTAT1034700.065.3535642
CGATCTC980000.065.3329638
CACGATC985100.065.2815536
TCGTATG1074850.065.2272443
TCTCGTA1003550.065.1669141
CGTATGC1082950.065.1425244
ACGATCT981450.065.12578637
TATGCCG1081450.065.10261546
ATGCCGT1082600.064.9034447
TCACGAT1030350.064.7918435
ATCACGA1065950.064.7557334
ATCTCGT993500.064.6935740
GCCGTCT1074600.064.6305849
TGCCGTC1084200.064.63010448
CCGTCTT1078950.064.3829950
CATCACG1100050.064.34511633
CGTCTTC1080700.064.0259251
GTATGCC1122850.062.7838745
CGTCTGA1163900.062.69617516
Produced by FastQC (version 0.11.5)