FastQCFastQC Report
Tue 17 Sep 2019
HFJFLBGXC_n01_mb262.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJFLBGXC_n01_mb262.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34431951
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC2956720.858714047310302TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG370700.063.50201443
CTCGTAT346100.063.29306442
TCTCGTA344100.063.20326641
CGTATGC379400.063.1710744
TATGCCG382100.062.53140646
ATGCCGT376800.062.33236747
AATCTCG353800.062.25024839
TGCCGTC377550.061.6311548
ATCTCGT358350.061.2163740
GCCGTCT365900.061.19224549
CCGTCTT373350.060.85225750
CGTCTTC374550.059.62927251
CGTCTGA477350.056.04414716
GTATGCC431150.055.88081745
ACGTCTG493300.054.31711215
ACACGTC502800.053.4164413
CACGTCT510600.052.63455614
GTAATCT433500.051.4668437
CACACGT544000.049.57072412
GCACACG545100.049.48267711
Produced by FastQC (version 0.11.5)