FastQCFastQC Report
Tue 17 Sep 2019
HFJFLBGXC_n01_mb261.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJFLBGXC_n01_mb261.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32459675
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC8307812.5594248864167617TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGC1152340.3550066351557741TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTTTGC495950.1527895766054343TruSeq Adapter, Index 7 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA157400.065.7611141
CGTCTGA1608600.065.71274616
TATGCCG1110350.065.53654546
ACACGTC1624150.065.4011713
TGCCGTC1056500.065.3939248
CATCTCG1139000.065.3075639
ACGTCTG1620650.065.302215
TCGTATG1001050.065.2876443
CGTATGC1106700.065.2633544
GCCGTCT958200.065.15819549
ATGCCGT1053150.065.1337247
CATCGCG225150.065.0781439
CACGTCT1630850.065.0463414
CTCGTAT1021150.065.0107542
ATCGCGT193150.064.91444440
ATCTCGT1096250.064.8884440
CCGTCTT989250.064.8501750
CGCGTAT164750.064.8456742
TCTCGTA1017000.064.8011541
GCACACG1648100.064.6194511
Produced by FastQC (version 0.11.5)