FastQCFastQC Report
Tue 17 Sep 2019
HFJFLBGXC_n01_mb260.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJFLBGXC_n01_mb260.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31216756
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC5367751.7195092276724717TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT616800.064.9634942
TCGTATG620450.064.8686443
CGCCAAT638150.064.8617933
TCTCGTA621100.064.6600641
TATCTCG624200.064.6458139
CGTATGC625400.064.60634644
TATGCCG624800.064.5383946
ACGCCAA646450.064.2127132
ATGCCGT625650.064.0415947
GTCACGC651000.063.90876429
TGCCGTC624150.063.86736748
ATCTCGT633400.063.7570940
GCCGTCT616450.063.67109349
CCGTCTT623200.063.30710250
TCACGCC657500.063.23438330
CACGCCA660150.062.94873431
CGTCTTC623250.062.9481251
AGTCACG664850.062.6933528
CGTCTGA676800.062.5660616
ACACGTC683250.062.36502513
Produced by FastQC (version 0.11.5)