FastQCFastQC Report
Tue 17 Sep 2019
HFJFLBGXC_n01_mb259.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJFLBGXC_n01_mb259.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29367606
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC4230371.4404885437376134TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC1633830.5563374828714333TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGC309400.1053541783419459TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC962700.064.96319613
CGTATGC759100.064.7998444
TCTCGTA509050.064.7873541
CTCGTAT518050.064.7567442
TCGTATG699350.064.750843
CGTCTGA960300.064.6769316
GATCTCG526150.064.6229239
TATGCCG762650.064.5521946
ACGTCTG968000.064.2817715
ATGCCGT737950.064.2458347
TGCCGTC733100.064.09106448
CACGTCT973600.064.0559314
GATCGGA1000800.063.906711
GCCGTCT686350.063.75422349
ATCGGAA1007700.063.7435262
CCGTCTT707800.063.6022250
ATCTCGT523950.063.55190740
GCACACG989350.063.37215411
CGTCTTC689700.063.27190451
TCGGAAG1013000.063.2270053
Produced by FastQC (version 0.11.5)