FastQCFastQC Report
Tue 17 Sep 2019
HFJFLBGXC_n01_mb258.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJFLBGXC_n01_mb258.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42468498
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC3286210.7737994407054377TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT412100.061.4696942
TCGTATG430300.061.3261143
TCTCGTA406700.061.29624641
AATCTCG405450.060.9574539
CGTATGC433650.060.89243344
ATGCCGT432750.060.62215847
TGCCGTC438500.059.68914448
TATGCCG441750.059.65682646
GCCGTCT434150.059.44869249
CCGTCTT440600.058.72133650
ATCTCGT423600.058.47809240
CGTCTTC443150.058.05164751
GTATGCC495300.053.36974745
ACCAATC510500.048.37902536
GACCAAT538850.047.83416435
GCTTGAA550000.046.554859
CCAATCT545000.045.5671437
CAATCTC548050.045.35186438
ACTGACC598250.045.0657932
TGCTTGA586550.043.2890658
Produced by FastQC (version 0.11.5)