FastQCFastQC Report
Tue 17 Sep 2019
HFJFLBGXC_n01_mb257.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJFLBGXC_n01_mb257.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44038452
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC5948781.3508149650673462TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC566740.12869208027566453TruSeq Adapter, Index 2 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGTA945500.065.1795334
CTCGTAT718450.065.1108642
TCTCGTA717000.064.9788441
TCGTATG705300.064.71654543
TATCTCG770450.064.70386539
ATGCCGT729300.064.60068547
CGTATGC758950.064.48106444
ACCGATG960550.064.4619732
CACCGAT963100.064.4220531
CCGATGT960200.064.2844633
TGCCGTC731300.064.00027548
TATGCCG772350.063.83273346
TCGCGTA74300.063.6472441
ATCTCGT760900.063.5886240
CCGTCTT708100.063.3632750
GTCACCG981450.063.3566429
GCCGTCT690100.063.13447649
TATCGCG99450.062.93125539
TCACCGA988050.062.92988630
CGCGTAT77400.062.3191942
Produced by FastQC (version 0.11.5)