FastQCFastQC Report
Tue 17 Sep 2019
HFJFLBGXC_n01_GG053.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJFLBGXC_n01_GG053.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33347747
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC1897320.5689499803390016TruSeq Adapter, Index 16 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG266650.062.61653543
GATCGGA355950.062.5973551
CTCGTAT248800.062.5509542
TCTCGTA234600.062.3830141
ATCGGAA360750.062.0068322
CGTATGC290800.061.9899344
ACCCGTC346500.060.87932232
ATGCCGT289300.060.66483347
TATGCCG298450.060.44734646
ACACGTC367300.059.7924613
CGTCTGA365900.059.6667916
CGTCCAT321550.059.5645235
CCGTCCA350550.059.1399834
TGCCGTC295450.058.9846648
ACGTCTG373900.058.58689515
CACCCGT360750.058.5424431
ATCTCGT255650.058.43669540
TCGGAAG383150.058.42753
TCACCCG364600.057.97234330
GCCGTCT279500.057.80476849
Produced by FastQC (version 0.11.5)