FastQCFastQC Report
Tue 17 Sep 2019
HFJFLBGXC_n01_GG052.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJFLBGXC_n01_GG052.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31387400
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGC1831560.5835335198200552TruSeq Adapter, Index 15 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT242250.058.5059542
TCGTATG253550.058.14834243
TCTCGTA240950.058.124241
CGTATGC257800.057.40688344
ATGCCGT261500.056.48583247
AATCTCG249000.055.8777139
GATCGGA289300.055.109031
TATGCCG269000.055.0422146
ATCGGAA290600.054.97092
ATCTCGT254250.054.90365640
TGCCGTC271500.054.2867848
CCGTCTT270900.053.81208450
GCCGTCT270500.053.63339249
ACACGTC295750.053.0163513
CGTCTGA293350.052.8767416
CGTCTTC282000.051.4333151
ACGTCTG303550.051.2613815
TCGGAAG313900.050.779373
GTATGCC297100.049.90735245
GTCAATC284200.049.5102636
Produced by FastQC (version 0.11.5)