FastQCFastQC Report
Tue 17 Sep 2019
HFJFLBGXC_n01_GG051.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJFLBGXC_n01_GG051.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31522730
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC3844371.2195549053016663TruSeq Adapter, Index 14 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATGTCGTATGC405110.12851361541338582TruSeq Adapter, Index 14 (97% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA617950.065.777711
ATCGGAA624500.065.2727662
CTCGTAT470800.064.3413542
TCGTATG522900.064.2499143
TCTCGTA456850.064.1834741
CGTATGC546650.063.9042344
ACACGTC627450.063.6946413
CGTCTGA629800.063.206216
ATGCCGT544400.062.99693747
TCGGAAG647000.062.8786243
TATGCCG559400.062.64107546
ATCTCGT471250.062.4659540
ACGTCTG638200.062.3963115
TGCCGTC549150.062.1057648
CGGAAGA655000.061.993474
GCCGTCT525500.061.4766949
CACGTCT651150.061.31173714
CCGTCTT532650.061.2423850
CATCTCG487750.060.71840739
CGTCTTC532550.059.8604551
Produced by FastQC (version 0.11.5)