FastQCFastQC Report
Tue 17 Sep 2019
HFJFLBGXC_n01_GG050.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJFLBGXC_n01_GG050.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35723576
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGC3916521.0963404111615254TruSeq Adapter, Index 13 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA524450.064.8167951
ATCGGAA528550.064.141832
TCGTATG493250.062.6257743
CTCGTAT473000.062.5324342
TCTCGTA470050.062.43356341
ACACGTC532400.062.3459913
CGTATGC499900.062.09341444
CGTCTGA536200.061.70145816
AATCTCG473450.061.63677239
TCGGAAG550550.061.4262283
ATGCCGT502850.061.3446647
TATGCCG509050.060.94869646
ACGTCTG544750.060.72035615
ATCTCGT484100.060.28827340
CGGAAGA560050.060.1843644
TGCCGTC511700.059.8716848
CACGTCT556200.059.59629414
GCCGTCT506850.059.5466149
CCGTCTT508100.059.5034550
CGTCTTC514250.058.26078851
Produced by FastQC (version 0.11.5)