FastQCFastQC Report
Wed 27 Sep 2017
HFJFLBGX3_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFJFLBGX3_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367198265
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGAG33278650.068.95158
CTCGAGG29572700.068.907059
GTGTCGG139024250.068.827746
TGTCGGT138463950.068.778117
TCGAGGT29797350.068.76060510
TCGGTCT138582400.068.7521749
GTCGGTC138528000.068.733198
TCCTCGA33449800.068.671627
CTCGTAG143105850.068.6633614
GGTCTCT125170800.068.6575314
GGTCTCG138733200.068.6269711
GTCTCGT140204100.068.6134312
ACGAGGT95759000.068.55617510
CGAGGTC125598500.068.5441611
GAGGTCT125529250.068.5435712
CGCCGGT4939200.068.538981
CCTGTGT10816950.068.53193
TCGTAGC34783750.068.5115
GTCCTCG33518200.068.48886
AGGTCTC125420350.068.4692813
Produced by FastQC (version 0.11.2)