FastQCFastQC Report
Thu 13 Jan 2022
HFHWLAFX3_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHWLAFX3_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8393701
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACCGCTGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTCT1106581.318345745220136Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCACTAGATCGGAAGAGCACACGTCT553020.6588512028245943Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGATATCGTTCAGCGGTTAGGATATGAGATCGGAAGAGCACACGTCTGA509390.6068717482312034Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGAGATCGGAAGAGCACACGTCT367550.4378878875957101Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCACAAGATCGGAAGAGCACACGTCT289550.34496106068109883Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACTTGGTTGATCCTGCCAAGGAAGCACACTTAGATCGGAAGAGCACACGT135950.16196669383386422No Hit
CAAGTGGGCTCTGACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC115800.137960596880923Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCACCGCTGGTCTAATGGTAGAATTTCACGAGATCGGAAGAGCACACGTCT113950.13575656316564053Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGACCATAGCTCAGTTGGAAGAGCATGAGATCGGAAGAGCACACGTCTGA111660.1330283268369936Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACCGCTGGTCTAATGGTAGAATTAGATCGGAAGAGCACACGTCTGAACT101940.12144821455994204Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TACATCTTTTTTAATCGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGT99000.11794558800700668Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCACCGCTGGTCTAATGGTAGAATTTAGATCGGAAGAGCACACGTCTGAAC89720.10688967834331958Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)