FastQCFastQC Report
Thu 13 Jan 2022
HFHWLAFX3_n01_920.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHWLAFX3_n01_920.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20123584
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACCGCTGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTCT15726907.815158572150965Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCACTAGATCGGAAGAGCACACGTCT13662536.7893124803215965Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGATATCGTTCAGCGGTTAGGATATGAGATCGGAAGAGCACACGTCTGA8333374.14109633751125Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGAGATCGGAAGAGCACACGTCT6766273.3623583154968815Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCACAAGATCGGAAGAGCACACGTCT4071192.023093898184339Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC3711501.8443533716459255TruSeq Adapter, Index 12 (100% over 50bp)
GCACCGCTGGTCTAATGGTAGAATTAGATCGGAAGAGCACACGTCTGAACT2236921.1115912553151566Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TACATCTTTTTTAATCGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGT2146981.066897427416508Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCGACCATAGCTCAGTTGGAAGAGCATGAGATCGGAAGAGCACACGTCTGA2118071.052531199213818Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC2087211.0371959587318045TruSeq Adapter, Index 12 (98% over 50bp)
TCCGATATCGTTCAGCGGTTAGGATATAAGATCGGAAGAGCACACGTCTGA1992980.9903703038186438Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGATATCGTTCAGCGGTTAGGATATGTAAGATCGGAAGAGCACACGTCT1871870.930187187332038Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTAGATCGGAAGAGCACACGTCTGAAC1799960.8944529960468274Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGATCGGAAGAGCACACGTCTG1549200.7698429862195522Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGATATCGTTCAGCGGTTAGGATATGTAGATCGGAAGAGCACACGTCTG1469080.7300290047737024Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGGAGATCGGAAGAGCACACGTC1311290.6516185188483323No Hit
TACTTGGTTGATCCTGCCAAGGAAGCACACTTAGATCGGAAGAGCACACGT1297200.6446167839685019No Hit
GCACTGCTGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTCT1186720.5897160267276446Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGCTGGTCTAATGGTAGAATTTCACTAGATCGGAAGAGCACACGTCT1031320.5124932020061636Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGTCATAGTGTAATGGTTATCAGATCGGAAGAGCACACGTCTGAACTCC1017700.5057250239321186Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTAGGATATGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT1015280.5045224548470093TruSeq Adapter, Index 12 (100% over 38bp)
CCGACCATAGCTCAGTTGGAAGAGCATAGATCGGAAGAGCACACGTCTGAA986680.49031027475026323Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTGACTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAT976550.4852763801915206TruSeq Adapter, Index 12 (100% over 41bp)
GCACCGCCGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTCT928620.4614585552951204Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATG928170.46123493707681495TruSeq Adapter, Index 12 (97% over 41bp)
CTTCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAT918670.45651410802369996TruSeq Adapter, Index 12 (100% over 41bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC918170.4562656433366939TruSeq Adapter, Index 12 (100% over 44bp)
TCCGATACCGTTCAGCGGTTAGGATATGTGAGATCGGAAGAGCACACGTCT896540.4455170609768121Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACCGCCGGTCTAATGGTAGAATTTCACTAGATCGGAAGAGCACACGTCT787070.391118202403707Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC781230.3882161348594763TruSeq Adapter, Index 12 (100% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGG763170.3792415903648177TruSeq Adapter, Index 12 (97% over 49bp)
CCGGACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAT695350.3455398402193168TruSeq Adapter, Index 12 (100% over 41bp)
GCACCGCCGGTCTAATGGTAGAATTAGATCGGAAGAGCACACGTCTGAACT667410.33165563350941857Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCACCGCCGGTCTAATGGTAGAATTTAGATCGGAAGAGCACACGTCTGAAC666040.330974840267022Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGAAGATCGGAAGAGCACACGT658070.32701431315614554No Hit
GTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTA652550.3242712630115987TruSeq Adapter, Index 12 (97% over 47bp)
TCCGATACCGTTCAGCGGTTAGGATATGAGATCGGAAGAGCACACGTCTGA652370.3241818157242765Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGGATATGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA634270.31518739405465745TruSeq Adapter, Index 12 (100% over 39bp)
GCCGGACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA586510.29145404715183937TruSeq Adapter, Index 12 (100% over 40bp)
GCTTGACTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAT535090.26590193874013696TruSeq Adapter, Index 12 (100% over 41bp)
GTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTA534430.265573965353289TruSeq Adapter, Index 12 (100% over 47bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATTG517860.2573398456259084TruSeq Adapter, Index 12 (97% over 48bp)
CCGACCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATG512040.25444771666915794TruSeq Adapter, Index 12 (97% over 41bp)
GTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTT486740.24187540350665168TruSeq Adapter, Index 12 (97% over 46bp)
GGGACTTTAGCTCACCTGGGAGAGCGTGCGAAGATCGGAAGAGCACACGTC484810.24091632981480832No Hit
CCGACCATAGCTCAGTTGGAAGAGCATAAGATCGGAAGAGCACACGTCTGA467260.2321952193008959Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACCGCTGGTCTAATGGTAGAATTTCAGATCGGAAGAGCACACGTCTGAA441060.21917566970177876Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACCGCTGGTCTAATGGTAGAATTTCACGAGATCGGAAGAGCACACGTCT437580.21744635548021665Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGATACCGTTCAGCGGTTAGGATATGTGGAGATCGGAAGAGCACACGTC436370.216845070937662No Hit
CGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTC433490.21541391434050713TruSeq Adapter, Index 12 (100% over 44bp)
GTCCCAGTAGTATATTGGTTAGAATAGATCAGATCGGAAGAGCACACGTCT429430.213396381082018Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTC411710.20459079257452348TruSeq Adapter, Index 12 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGG405550.20152970763060896TruSeq Adapter, Index 12 (97% over 49bp)
CGTGTCGGAACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG403810.20066505051982786TruSeq Adapter, Index 12 (100% over 37bp)
GCACCGCTGGTCTAATGGTAGAATCAGATCGGAAGAGCACACGTCTGAACT403800.20066008122608778Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGAAGATCGGAAGAGCACACGTC398830.1981903422372476No Hit
CGGACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATC394870.19622250191615964TruSeq Adapter, Index 12 (100% over 42bp)
TCCGACATCGTTCATCGGTTAGGATAAGCGAGATCGGAAGAGCACACGTCT382010.189831990166364Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGATATCGTTCAGCGGTTAGGATAAGATCGGAAGAGCACACGTCTGAAC378340.18800825936373958Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT374500.1861000505675331TruSeq Adapter, Index 12 (100% over 43bp)
GGGACTTTAGCTCACCTGGGAGAGCGTGAGATCGGAAGAGCACACGTCTGA354280.17605213862500835Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTC345220.17154995849645868TruSeq Adapter, Index 12 (97% over 44bp)
GCCTGTGCAGCTCAACCGGATAGAGCATAAGATCGGAAGAGCACACGTCTG337950.1679372819473907Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGTCACAGTGTAATGGTTATCAGATCGGAAGAGCACACGTCTGAACTCC334630.1662874764256705Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATTC330580.16427491246092146TruSeq Adapter, Index 12 (97% over 48bp)
GCACTGCTGGTCTAATGGTAGAATTTAGATCGGAAGAGCACACGTCTGAAC307960.15303437002076767Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGCTGGTCTAATGGTAGAATTTCACAAGATCGGAAGAGCACACGTCT298480.14832347955513292Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCAAGATCGGAAGAGCACACGTCTGA297140.14765759419395671Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGACCACAGCTCAGTTGGAAGAGCATGAGATCGGAAGAGCACACGTCTGA296530.1473544672758093Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGATATCGTTCAGCGGTTAGGATATGCAGATCGGAAGAGCACACGTCTG291930.14506859215535364Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACCGCTGGTCTAATGGTAGAATTCAGATCGGAAGAGCACACGTCTGAAC284780.1415155471311671Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGT284410.14133168326278261TruSeq Adapter, Index 12 (97% over 43bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAAGATCGGAAGAGCACACGTCT276440.13737115615190615Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGAGATCGGAAGAGCACACGTC265060.1317160998756484No Hit
TACTTGGTTGATCCTGCCAAGGAAGCACACTCAGATCGGAAGAGCACACGT260830.1296140886235772No Hit
TTAGGATATGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT260700.12954948780495562TruSeq Adapter, Index 12 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATTC253940.12619024523663377TruSeq Adapter, Index 12 (98% over 50bp)
CCGACCACAGCTCAGTTGGAAGAGCATAGATCGGAAGAGCACACGTCTGAA249740.12410314186578296Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCTGTGCAGCTCAACCGGATAGAGCATAAGAAGATCGGAAGAGCACACGT246280.12238376623170107No Hit
CCGTGTCGGAACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT239830.119178571769323TruSeq Adapter, Index 12 (100% over 36bp)
TCGGTCATAGTGTAATGGTTATTAGATCGGAAGAGCACACGTCTGAACTCC239010.11877108968263307Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACCGCCGGTCTAATGGTAGAATTTCACAAGATCGGAAGAGCACACGTCT237670.11810520432145685Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGATACCGTTCAGCGGTTAGGATATGTAAGATCGGAAGAGCACACGTCT237490.11801575703413467Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGACTTTAGCTCACCTGGGAGAGCGTGCGAGATCGGAAGAGCACACGTCT237130.11783686245949031Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT235890.1172206700357153TruSeq Adapter, Index 12 (100% over 43bp)
GGGACTTTAGCTCACCTGGGAGAGCGTGCAGATCGGAAGAGCACACGTCTG235240.11689766594260745Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGCTGGTCTAATGGTAGAATTAGATCGGAAGAGCACACGTCTGAACT232850.11571000473871852Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT226940.11277315213830698TruSeq Adapter, Index 12 (100% over 43bp)
GCTTTCCCAGCTCAACAGGATAGAGCTTTGGAAGATCGGAAGAGCACACGT225040.11182898632768397No Hit
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTG221930.11028353597450632TruSeq Adapter, Index 12 (97% over 43bp)
CCGACCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATC216290.10748085430507806TruSeq Adapter, Index 12 (100% over 42bp)
CCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCT212490.10559252268383205TruSeq Adapter, Index 12 (100% over 43bp)
GCACCGCTGGTCTAATGGTAGAATTTCACAGATCGGAAGAGCACACGTCTG210140.10442473865490362Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)