FastQCFastQC Report
Thu 13 Jan 2022
HFHWLAFX3_n01_919.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHWLAFX3_n01_919.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19716011
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACCGCTGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTCT15030467.623479211895347Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGATATCGTTCAGCGGTTAGGATATGAGATCGGAAGAGCACACGTCTGA9773344.95705748997604Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGAGATCGGAAGAGCACACGTCT6601063.3480707634013798Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCACTAGATCGGAAGAGCACACGTCT6445703.2692718623457857Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCACAAGATCGGAAGAGCACACGTCT2121381.0759681560331853Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC2042761.036091935635459TruSeq Adapter, Index 7 (100% over 50bp)
GCACCGCTGGTCTAATGGTAGAATTAGATCGGAAGAGCACACGTCTGAACT1767640.8965505243428805Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGACCATAGCTCAGTTGGAAGAGCATGAGATCGGAAGAGCACACGTCTGA1746670.8859144986275368Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACCGCTGGTCTAATGGTAGAATTTAGATCGGAAGAGCACACGTCTGAAC1586190.8045187233867946Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGATCGGAAGAGCACACGTCTG1578980.8008617970440369Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT1522600.7722657488880483TruSeq Adapter, Index 7 (100% over 41bp)
GCACCGCCGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTCT1517720.7697906031803289Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGATATCGTTCAGCGGTTAGGATATGTAGATCGGAAGAGCACACGTCTG1424180.7223469291024437Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGCTGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTCT1424160.722336785062658Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACATCTTTTTTAATCGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGT1371910.6958354811224238Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCGATATCGTTCAGCGGTTAGGATATAAGATCGGAAGAGCACACGTCTGA1347930.6836727774193269Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATC1253030.6355393086360116TruSeq Adapter, Index 7 (100% over 42bp)
CCGGACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT1215600.6165547381770076TruSeq Adapter, Index 7 (100% over 41bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGGAGATCGGAAGAGCACACGTC1215350.6164279376796858No Hit
TCCGATATCGTTCAGCGGTTAGGATATGTAAGATCGGAAGAGCACACGTCT1132690.5745026212452408Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGAAGATCGGAAGAGCACACGT1095150.5554622585674152No Hit
TTAGGATATGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT1087930.5518002602047646TruSeq Adapter, Index 7 (100% over 38bp)
GCTTGACTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT1084250.5499337568841892TruSeq Adapter, Index 7 (100% over 41bp)
TCGGTCATAGTGTAATGGTTATCAGATCGGAAGAGCACACGTCTGAACTCC1062830.5390694902736664Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATG914310.46373985082479413TruSeq Adapter, Index 7 (97% over 41bp)
TACTTGGTTGATCCTGCCAAGGAAGCACACTTAGATCGGAAGAGCACACGT863000.43771531675448955No Hit
TCCGATACCGTTCAGCGGTTAGGATATGTGAGATCGGAAGAGCACACGTCT842320.42722637961603893Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGG780580.3959117287974733TruSeq Adapter, Index 7 (97% over 49bp)
TCCGATACCGTTCAGCGGTTAGGATATGTGGAGATCGGAAGAGCACACGTC759490.3852148388434151No Hit
GGGACTTTAGCTCACCTGGGAGAGCGTGCGAAGATCGGAAGAGCACACGTC733960.3722659720569237No Hit
TAGGATATGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC727010.3687409182313806TruSeq Adapter, Index 7 (100% over 39bp)
CGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCG719360.3648608230133367TruSeq Adapter, Index 7 (97% over 42bp)
CCGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCG715790.36305011191158293TruSeq Adapter, Index 7 (97% over 42bp)
GCACCGCCGGTCTAATGGTAGAATTTAGATCGGAAGAGCACACGTCTGAAC688240.34907669710673217Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC677050.3434011068466131TruSeq Adapter, Index 7 (100% over 44bp)
GCACCGCCGGTCTAATGGTAGAATTAGATCGGAAGAGCACACGTCTGAACT667290.33845081543117417Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTA661610.33556990813202525TruSeq Adapter, Index 7 (100% over 47bp)
GCACCGCCGGTCTAATGGTAGAATTTCACTAGATCGGAAGAGCACACGTCT647070.3281951912077955Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGATACCGTTCAGCGGTTAGGATATGAGATCGGAAGAGCACACGTCTGA645970.32763726901958007Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGCTGGTCTAATGGTAGAATTTCACTAGATCGGAAGAGCACACGTCT609530.3091548285299699Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTT590900.2997056554695572TruSeq Adapter, Index 7 (97% over 46bp)
GCCTGTGCAGCTCAACCGGATAGAGCATAAGATCGGAAGAGCACACGTCTG581730.29505461322779747Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCTGTGCAGCTCAACCGGATAGAGCATAAGAAGATCGGAAGAGCACACGT573640.2909513491344674No Hit
GCCGGACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA568990.2885928598842839TruSeq Adapter, Index 7 (100% over 40bp)
GCACCGCTGGTCTAATGGTAGAATTTCAGATCGGAAGAGCACACGTCTGAA564730.2864321794099222Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTGTCGGAACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA533170.2704248846280315TruSeq Adapter, Index 7 (100% over 37bp)
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC528580.2680968274972052TruSeq Adapter, Index 7 (100% over 44bp)
GCACCGCTGGTCTAATGGTAGAATTTCAAGATCGGAAGAGCACACGTCTGA485090.24603861298312318Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGTCACAGTGTAATGGTTATCAGATCGGAAGAGCACACGTCTGAACTCC482800.2448771204276565Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGTCTCTAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT457910.23225286291430858TruSeq Adapter, Index 7 (100% over 38bp)
CCGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT452290.22940238773451688TruSeq Adapter, Index 7 (100% over 43bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTG451310.22890532978501582TruSeq Adapter, Index 7 (97% over 43bp)
CGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT441610.22398547048893408TruSeq Adapter, Index 7 (100% over 43bp)
CCGACCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATC438210.22226098372535907TruSeq Adapter, Index 7 (100% over 42bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGG423800.21495220305973659TruSeq Adapter, Index 7 (97% over 42bp)
CCGACCATAGCTCAGTTGGAAGAGCATAGATCGGAAGAGCACACGTCTGAA412960.2094541334958679Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGACTTTAGCTCACCTGGGAGAGCGTGAGATCGGAAGAGCACACGTCTGA385740.19564809534748182Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGAGATCGGAAGAGCACACGTC369120.18721839828553555No Hit
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTG347760.17638456379436995TruSeq Adapter, Index 7 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTGGTATTG338270.17157121691603844TruSeq Adapter, Index 7 (97% over 48bp)
GCACTGCCGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTCT332800.16879682203463975Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTTCCCAGCTCAACAGGATAGAGCTTTGGAAGATCGGAAGAGCACACGT329780.1672650720269937No Hit
GCACCGCTGGTCTAATGGTAGAATTTCACAGATCGGAAGAGCACACGTCTG328270.16649919702317065Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGACTTTAGCTCACCTGGGAGAGCGTGCAGATCGGAAGAGCACACGTCTG328190.1664586208640277Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGACCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATG323950.16430808442945177TruSeq Adapter, Index 7 (97% over 41bp)
GGGACTTTAGCTCACCTGGGAGAGCGTGCGAGATCGGAAGAGCACACGTCT323400.16402912333534403Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTGGTATGG322540.16359292962455743TruSeq Adapter, Index 7 (97% over 49bp)
TCCGACATCGTTCATCGGTTAGGATAAGCGAGATCGGAAGAGCACACGTCT321040.16283212664062727Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGG319330.16196481123894685TruSeq Adapter, Index 7 (97% over 42bp)
CCGTGTCGGAACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG310940.1577093865488308TruSeq Adapter, Index 7 (100% over 36bp)
GTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTGGTT302580.15346917791839332TruSeq Adapter, Index 7 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATTC294000.14911738485031278TruSeq Adapter, Index 7 (98% over 50bp)
GTCCCAGTAGTATATTGGTTAGAATAGATCAGATCGGAAGAGCACACGTCT288230.14619082937212807Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGATATCGTTCAGCGGTTAGGATAAGATCGGAAGAGCACACGTCTGAAC285280.14469458350373207Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCG282780.14342657853051513TruSeq Adapter, Index 7 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGTATTG278490.14125068199647484TruSeq Adapter, Index 7 (97% over 42bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAAGATCGGAAGAGCACACGTCT270990.13744666707682401Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTGACTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT270680.1372894344601451TruSeq Adapter, Index 7 (100% over 41bp)
CTTCGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT267450.13565117203474883TruSeq Adapter, Index 7 (100% over 41bp)
CCGACCACAGCTCAGTTGGAAGAGCATGAGATCGGAAGAGCACACGTCTGA262120.1329477854318503Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTCTGAACTC261700.13273476059634984Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGGGCGGTGCACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA253830.1287430809406629TruSeq Adapter, Index 7 (100% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATTG249500.12654689632705113TruSeq Adapter, Index 7 (97% over 48bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGAAGATCGGAAGAGCACACGTC241680.12258057677082854No Hit
GCACCGCTGGTCTAATGGTAGAATCAGATCGGAAGAGCACACGTCTGAACT239490.1214698044142905Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGATATCGTTCAGCGGTTAGGATATGCAGATCGGAAGAGCACACGTCTG238330.12088145010671783Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGACTTCAGCTCACCTGGGAGAGCGTGCGAAGATCGGAAGAGCACACGTC232600.1179751827081046No Hit
CCGACCATAGCTCAGTTGGAAGAGCATAAGATCGGAAGAGCACACGTCTGA231840.11758970919624664Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGCTGGTCTAATGGTAGAATTTAGATCGGAAGAGCACACGTCTGAAC225110.11417623980834662Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATGG220620.11189890287644899TruSeq Adapter, Index 7 (97% over 41bp)
GCACCGCTGGTCTAATGGTAGAATTTCACGAGATCGGAAGAGCACACGTCT215940.10952519756658687Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCG213680.10837892107079874TruSeq Adapter, Index 7 (97% over 42bp)
GGTTAGGATATGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG212740.10790215120086918TruSeq Adapter, Index 7 (100% over 36bp)
CCGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATGG211010.10702469175940307TruSeq Adapter, Index 7 (97% over 41bp)
CGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC210830.10693339540133143TruSeq Adapter, Index 7 (100% over 44bp)
CCGGACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAT210230.10662907420775937TruSeq Adapter, Index 7 (100% over 41bp)
GCACCGCCGGTCTAATGGTAGAATTTCACAAGATCGGAAGAGCACACGTCT208820.10591391940286501Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATC206720.10484879522536278TruSeq Adapter, Index 7 (100% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTGGTATGC205280.1041184243607898TruSeq Adapter, Index 7 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGTTTTG203300.103114164422002TruSeq Adapter, Index 7 (97% over 42bp)
CCGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCG202470.10269318677089397TruSeq Adapter, Index 7 (97% over 42bp)
TCCGATACCGTTCAGCGGTTAGGATATGTAGATCGGAAGAGCACACGTCTG198750.10080639537074715Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)