FastQCFastQC Report
Thu 13 Jan 2022
HFHWLAFX3_n01_918.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHWLAFX3_n01_918.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18685772
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC17771419.510664049630916TruSeq Adapter, Index 6 (100% over 50bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTCT16666308.919246151563874Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGATATCGTTCAGCGGTTAGGATATGAGATCGGAAGAGCACACGTCTGA7240303.8747663195291047Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACCGCTGGTCTAATGGTAGAATTTCACTAGATCGGAAGAGCACACGTCT5721213.0618001760911993Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGAGATCGGAAGAGCACACGTCT4865342.603767187141104Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACTTGGTTGATCCTGCCAAGGAAGCACACTTAGATCGGAAGAGCACACGT2861691.531480743744492No Hit
CCGACCATAGCTCAGTTGGAAGAGCATGAGATCGGAAGAGCACACGTCTGA2294091.2277202140751797Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTA2190121.1720789486246541TruSeq Adapter, Index 6 (100% over 47bp)
GCACCGCTGGTCTAATGGTAGAATTTCACAAGATCGGAAGAGCACACGTCT1937051.0366443516489445Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTAGATCGGAAGAGCACACGTCTGAAC1806810.9669442611201722Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACCGCTGGTCTAATGGTAGAATTAGATCGGAAGAGCACACGTCTGAACT1764650.9443816396775044Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGG1731270.9265177804802498TruSeq Adapter, Index 6 (97% over 49bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGGAGATCGGAAGAGCACACGTC1675840.8968534990151865No Hit
GCACTGCTGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTCT1505310.8055915484787035Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACATCTTTTTTAATCGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGT1376700.7367637794146262Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCGATATCGTTCAGCGGTTAGGATATGTAGATCGGAAGAGCACACGTCTG1337770.7159297459050662Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGATCGGAAGAGCACACGTCTG1249590.6687387601646858Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACCGCCGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTCT1238370.6627341915549435Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATC1042010.5576488892190271TruSeq Adapter, Index 6 (100% over 42bp)
CTTCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAT998250.5342300013079471TruSeq Adapter, Index 6 (100% over 41bp)
TTAGGATATGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA956940.5121222714266235TruSeq Adapter, Index 6 (100% over 38bp)
CGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC875190.4683724065561755TruSeq Adapter, Index 6 (100% over 44bp)
TCGGTCATAGTGTAATGGTTATCAGATCGGAAGAGCACACGTCTGAACTCC868760.464931285686243Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGATATCGTTCAGCGGTTAGGATATAAGATCGGAAGAGCACACGTCTGA826540.4423365542510098Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGACCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATC815990.4366905472249153TruSeq Adapter, Index 6 (100% over 42bp)
CGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC743820.3980675778340868TruSeq Adapter, Index 6 (100% over 44bp)
TCCGATATCGTTCAGCGGTTAGGATATGTAAGATCGGAAGAGCACACGTCT712080.38108139176695505Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGATACCGTTCAGCGGTTAGGATATGTGAGATCGGAAGAGCACACGTCT705540.37758140257731926Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGAAGATCGGAAGAGCACACGT699290.37423661168508315No Hit
CCGGACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAT684250.36618770688200625TruSeq Adapter, Index 6 (100% over 41bp)
AAGTCTCTAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA672540.35992090666631277TruSeq Adapter, Index 6 (100% over 38bp)
CCGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT656300.35122980201192655TruSeq Adapter, Index 6 (100% over 43bp)
GCACCGCTGGTCTAATGGTAGAATTTCAGATCGGAAGAGCACACGTCTGAA625550.334773430822125Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATTC625110.33453795754331156TruSeq Adapter, Index 6 (98% over 50bp)
TAGGATATGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAT619020.3312787932979167TruSeq Adapter, Index 6 (100% over 39bp)
TCCGATACCGTTCAGCGGTTAGGATATGTGGAGATCGGAAGAGCACACGTC590880.316219206784713No Hit
TCCGATACCGTTCAGCGGTTAGGATATGAGATCGGAAGAGCACACGTCTGA587730.314533432175026Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT576630.30859308355041476TruSeq Adapter, Index 6 (100% over 43bp)
GCACCGCCGGTCTAATGGTAGAATTTAGATCGGAAGAGCACACGTCTGAAC530320.28380952095530226Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTTGACTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAT526820.28193643805565005TruSeq Adapter, Index 6 (100% over 41bp)
GCACTGCTGGTCTAATGGTAGAATTTCACTAGATCGGAAGAGCACACGTCT508090.27191276870979697Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGTCGGAACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCA492220.2634196756762311TruSeq Adapter, Index 6 (100% over 37bp)
GGGACTTTAGCTCACCTGGGAGAGCGTGCGAAGATCGGAAGAGCACACGTC462330.24742354771320127No Hit
GCACCGCCGGTCTAATGGTAGAATTAGATCGGAAGAGCACACGTCTGAACT461530.24699541447899503Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTA451060.24139222077632114TruSeq Adapter, Index 6 (100% over 47bp)
TCCGACATCGTTCATCGGTTAGGATAAGCGAGATCGGAAGAGCACACGTCT449690.24065904261274298Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACCGCTGGTCTAATGGTAGAATTTCACAGATCGGAAGAGCACACGTCTG439280.23508795890263456Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTT424680.22727452737837112TruSeq Adapter, Index 6 (97% over 46bp)
GCCGGACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATA421170.22539609281329132TruSeq Adapter, Index 6 (100% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATTG417040.2231858549917017TruSeq Adapter, Index 6 (97% over 48bp)
GCACCGCCGGTCTAATGGTAGAATTTCACTAGATCGGAAGAGCACACGTCT416930.22312698667199837Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACTTGGTTGATCCTGCCAAGGAAGCACACTCAGATCGGAAGAGCACACGT397320.21263237076851843No Hit
CCGACCATAGCTCAGTTGGAAGAGCATAGATCGGAAGAGCACACGTCTGAA396230.21204903923691246Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACCGCTGGTCTAATGGTAGAATTTCAAGATCGGAAGAGCACACGTCTGA389840.20862932502869028Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCCCAGTAGTATATTGGTTAGAATAGATCAGATCGGAAGAGCACACGTCT386000.2065742855045004Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTA382660.20478682925168948TruSeq Adapter, Index 6 (100% over 47bp)
CCGACCACAGCTCAGTTGGAAGAGCATGAGATCGGAAGAGCACACGTCTGA327210.17511184445577094Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCTGTGCAGCTCAACCGGATAGAGCATAAGAAGATCGGAAGAGCACACGT323310.17302469493901562No Hit
CCGGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT321620.172120263481755TruSeq Adapter, Index 6 (100% over 43bp)
GCCTGTGCAGCTCAACCGGATAGAGCATAAGATCGGAAGAGCACACGTCTG306390.1639696770355541Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGTCACAGTGTAATGGTTATCAGATCGGAAGAGCACACGTCTGAACTCC304710.16307059724372105Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTA299540.16030378621766336TruSeq Adapter, Index 6 (100% over 47bp)
GCCTGTGTAGCTCAACCGGATAGAGCATAAGAGATCGGAAGAGCACACGTC295800.1583022633477493No Hit
CGGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT291850.1561883555038561TruSeq Adapter, Index 6 (100% over 43bp)
TCCGATATCGTTCAGCGGTTAGGATAAGATCGGAAGAGCACACGTCTGAAC290320.1553695506934367Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGAAGATCGGAAGAGCACACGTC281720.15076711842571983No Hit
CCGTGTCGGAACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC270140.14456988986058483TruSeq Adapter, Index 6 (100% over 36bp)
CCGACCATAGCTCAGTTGGAAGAGCATAAGATCGGAAGAGCACACGTCTGA251450.13456762717644205Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTA245790.13153858454443304TruSeq Adapter, Index 6 (100% over 47bp)
AAGTCTCTAAGCAATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG245360.13130846293104723Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCACTGCTGGTCTAATGGTAGAATTTAGATCGGAAGAGCACACGTCTGAAC242620.12984210660389092Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGCCGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTCT237920.1273268238529294Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGACAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC236640.12664181067819943TruSeq Adapter, Index 6 (100% over 44bp)
GGGACTTTAGCTCACCTGGGAGAGCGTGAGATCGGAAGAGCACACGTCTGA235630.1261012924700141Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTCTGAACTC229490.12281536989748136Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTTTCCCAGCTCAACAGGATAGAGCTTTGGAAGATCGGAAGAGCACACGT227850.1219376967673586No Hit
GCCTGTGTAGCTCAACCGGATAGAGCATAAAGATCGGAAGAGCACACGTCT227800.12191093844022072Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGACTTTAGCTCACCTGGGAGAGCGTGCGAGATCGGAAGAGCACACGTCT223540.11963112896807261Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT218550.11696064791971131TruSeq Adapter, Index 6 (100% over 43bp)
CGGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTC215850.11551569825426532TruSeq Adapter, Index 6 (100% over 44bp)
CCGGGCGGTGCACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC208100.11136815754789259TruSeq Adapter, Index 5 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTAAGC206540.11053329774119046TruSeq Adapter, Index 6 (98% over 50bp)
GGGACTTTAGCTCACCTGGGAGAGCGTGCAGATCGGAAGAGCACACGTCTG206280.11039415444007343Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGATACCGTTCAGCGGTTAGGATATGTAGATCGGAAGAGCACACGTCTG205910.11019614281925308Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACCGCTGGTCTAATGGTAGAATCAGATCGGAAGAGCACACGTCTGAACT205320.10988039455902597Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGTCTCTAAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAA199280.10664798864076902TruSeq Adapter, Index 6 (100% over 38bp)
TCCGATATCGTTCAGCGGTTAGGATATGCAGATCGGAAGAGCACACGTCTG195570.10466252076713768Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCT193050.10331390107938809TruSeq Adapter, Index 6 (100% over 43bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)