FastQCFastQC Report
Thu 13 Jan 2022
HFHWLAFX3_n01_830_small_RNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHWLAFX3_n01_830_small_RNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25067751
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTGGGCTCTGACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4338261.7306139669250744Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTGGGCTCTGACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC1993080.7950773086903568TruSeq Adapter, Index 5 (100% over 35bp)
CGGACTGCCGGAGAAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1810280.722154931250115Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGTGGGCTCTGACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1595740.6365708674862774Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAAGTGGGCTCTGACCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1179920.4706924047554167Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGTCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA1171780.4674452047971915TruSeq Adapter, Index 5 (100% over 40bp)
ACGGGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAT1134600.4526133995825952TruSeq Adapter, Index 5 (100% over 41bp)
AGGACTGCGAGCCTGAGAGGGTGCAGATCGGAAGAGCACACGTCTGAACTC1131300.4512969671670985Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAAGTGGGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC962360.3839036058719428Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAAGTGGGCTCTGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA925630.36925131416855067Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTCGATCAAGGGCGAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC797430.31810990942107253Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAGGTGCGTGAGGATTGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAG756730.3018739096299465Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGGGGAGAGTCGGATACTTTGAGAGTAGATCGGAAGAGCACACGTCTGAA752660.3002503096508338Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCAGCAGCAACTCTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC663450.2646627533519062Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTCT622470.2483150562649198Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGCTCTGACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA615000.2453351319789318TruSeq Adapter, Index 5 (100% over 36bp)
GCAGCAACTCTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA609110.24298549957672708TruSeq Adapter, Index 5 (100% over 36bp)
AGTGGGCTCTGACCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA587790.2344805483347908Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGGACTGCCGGAGAAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC533080.2126556945615105Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGGGAAATGTAGTCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC516570.2060695432948891Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAT507930.20262288387977045TruSeq Adapter, Index 5 (100% over 41bp)
AGGGTCGGATTGTTCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA477320.19041197592875403Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCAACTCTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG464920.18546538139779672TruSeq Adapter, Index 5 (100% over 39bp)
AGTGGGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC438600.17496583558692602TruSeq Adapter, Index 5 (100% over 35bp)
CGGACTGCCGGAGAAGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA429910.17149923022611802Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGTGCGTGAGGATTGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC398770.15907689525079455Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAGGAAGGAGAAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC392400.1565357817699721TruSeq Adapter, Index 5 (100% over 35bp)
AGGACAGAAATCTCACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA392280.15648791150031768Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGTGGGCTCTGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC382310.15251068992986247TruSeq Adapter, Index 5 (100% over 35bp)
GGGGAAACTCTGATGGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC379090.15122617102746871Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGTGGGCTCTGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA377460.15057593319799611TruSeq Adapter, Index 5 (100% over 36bp)
CGGGGAGAGTCGGATACTTTGAGAGTAGATCGGAAGAGCACACGTCTGAAC369940.14757606296631876Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGTGGGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA353400.14097794413228376Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TACTTGGTTGATCCTGCCAAGGAAGCACACTTAGATCGGAAGAGCACACGT351170.140088354954539No Hit
AGGACTGCGAGCCTGAGAGGGTGTAGATCGGAAGAGCACACGTCTGAACTC346460.1382094468706028Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGGAAGGAGAAGTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA343850.13716826850561903Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGTGGGCTCTGACCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC338410.1349981496146184Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGGTTACCGTCGGACTGCCGGAGAAGGCAGATCGGAAGAGCACACGTCTG333900.13319902531343958Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAAGTGGGCTCTGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA317260.12656101458802588Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGCTGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA316580.1262897497266508TruSeq Adapter, Index 5 (100% over 40bp)
CAAGTGGGCTCTGACCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC308780.12317818219911311Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGGGAACGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG303710.12115566330621362TruSeq Adapter, Index 5 (100% over 39bp)
GTTCTTGGGCTCTGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA291470.1162728958014622Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGTCGGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG287490.11468519852459041TruSeq Adapter, Index 5 (100% over 39bp)
CGCAAGGCTGAAACTTGAAGGAAGATCGGAAGAGCACACGTCTGAACTCCA284300.11341264718961028Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTCTTGGGCTCTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC274500.10950324183449883Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTTCTTGGGCTCTGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC262770.10482392297577874Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTGACTGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA250860.10007279871257696TruSeq Adapter, Index 5 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)