FastQCFastQC Report
Thu 13 Jan 2022
HFHWLAFX3_n01_829_small_RNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHWLAFX3_n01_829_small_RNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23496416
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTGGGCTCTGACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3882941.6525669276539878Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTGGGCTCTGACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG2016040.8580202189133866TruSeq Adapter, Index 2 (97% over 35bp)
AAGTGGGCTCTGACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1860000.7916100906623376Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGACTGCCGGAGAAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1601980.6817975984081998Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1309230.5572041284934689TruSeq Adapter, Index 4 (100% over 50bp)
GCACCGCTGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTCT1222740.5203942592776702Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGACTGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA776520.3304844449468379TruSeq Adapter, Index 4 (100% over 40bp)
CAAGTGGGCTCTGACCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA751050.31964449386663907Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAAGTGGGCTCTGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT693420.2951173489607947Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGACTGCGAGCCTGAGAGGGTGCAGATCGGAAGAGCACACGTCTGAACTC677790.2884652706182934Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA667380.28403480769152195TruSeq Adapter, Index 4 (100% over 40bp)
CAAGTGGGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC614660.261597343186297Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAGGTGCGTGAGGATTGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAG604640.25733286302047087Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGATGAAGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC579210.24650993581318953TruSeq Adapter, Index 4 (100% over 37bp)
GGGGAAGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC567470.2415134291119122TruSeq Adapter, Index 4 (100% over 37bp)
GTCGATCAAGGGCGAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC528500.22492792092206745Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAGTGGGCTCTGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG526880.2242384540689099TruSeq Adapter, Index 2 (97% over 35bp)
ACGGGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT516620.21987183066557894TruSeq Adapter, Index 4 (100% over 41bp)
GTGGGCTCTGACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA482970.20555049757375762TruSeq Adapter, Index 2 (97% over 36bp)
ACAAGTGGGCTCTGACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA475560.2023968251157964Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TACTTGGTTGATCCTGCCAAGGAAGCACACTTAGATCGGAAGAGCACACGT457090.1945360517961548No Hit
CCGGGGAGAGTCGGATACTTTGAGAGTAGATCGGAAGAGCACACGTCTGAA432890.18423660868108566Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGACTGCCGGAGAAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC425020.18088716168457353Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATGAAGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA408220.17373713505923627TruSeq Adapter, Index 4 (100% over 39bp)
AGTGGGCTCTGACCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT401990.17108566685234036Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGGACGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT383080.1630376309306066TruSeq Adapter, Index 4 (100% over 41bp)
ATGAAGGACGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC376470.16022443593099475TruSeq Adapter, Index 4 (100% over 38bp)
AGTGGGCTCTGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA345220.1469245352142216TruSeq Adapter, Index 2 (97% over 36bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGAGATCGGAAGAGCACACGTCT320750.13651018095695958Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGGGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG318550.13557386794649873TruSeq Adapter, Index 2 (97% over 35bp)
TACATCTTTTTTAATCGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGT303720.12926226706234686Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAGTGGGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT294370.12528293676788835Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT289150.12306132135215855TruSeq Adapter, Index 4 (100% over 41bp)
CTTTTGGTACTGTGGATAGGGGTAGATCGGAAGAGCACACGTCTGAACTCC273560.11642626688257478Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGACTGCCGGAGAAGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA262460.11170214214797694Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCGATATCGTTCAGCGGTTAGGATATGAGATCGGAAGAGCACACGTCTGA260130.11071050155053434Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGTGGGCTCTGACCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC256970.10936561559005424Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCAACTCTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA253090.10771429991705969TruSeq Adapter, Index 4 (100% over 39bp)
ACAAGTGGGCTCTGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC252220.10734403068110472Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGTGCGTGAGGATTGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC239880.10209216588606534Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGGAGAGTCGGATACTTTGAGAGTAGATCGGAAGAGCACACGTCTGAAC235360.10016846824639128Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)