FastQCFastQC Report
Thu 13 Jan 2022
HFHWLAFX3_n01_827_small_RNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHWLAFX3_n01_827_small_RNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25397478
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTGGGCTCTGACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2714451.0687872236763036Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGACTGCCGGAGAAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2347980.9244933689872672Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGTGGGCTCTGACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG1714830.6751969624700531TruSeq Adapter, Index 2 (100% over 35bp)
AAGTGGGCTCTGACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1642890.6468713153329634Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TACTTGGTTGATCCTGCCAAGGAAGCACACTTAGATCGGAAGAGCACACGT1532070.6032370615696566No Hit
GCACCGCTGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTCT1410860.5555118504286134Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGACTGCGAGCCTGAGAGGGTGCAGATCGGAAGAGCACACGTCTGAACTC1250640.4924268464766462Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGAAGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT1034060.4071506627547822TruSeq Adapter, Index 2 (100% over 37bp)
TACATCTTTTTTAATCGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGT870910.34291200094749563Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAGGTGCGTGAGGATTGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAG821830.3235872475211909Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCGATCAAGGGCGAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC747870.2944662458217308Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAGTGGGCTCTGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG642160.2528440028572916TruSeq Adapter, Index 2 (100% over 35bp)
CAAGTGGGCTCTGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC611070.24060262991467105Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGGACTGCCGGAGAAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC582170.22922354731442232Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAGTGGGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC549870.21650574911414433Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACAAGTGGGCTCTGACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA548200.21584820351060055Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGTCGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA513200.2020673076279464TruSeq Adapter, Index 2 (100% over 40bp)
CCGGGGAGAGTCGGATACTTTGAGAGTAGATCGGAAGAGCACACGTCTGAA500060.19689356557371562Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGACTGCCGGAGAAGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA499470.1966612590431223Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGATGAAGGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT495230.19499180194190935TruSeq Adapter, Index 2 (100% over 37bp)
CAAGTGGGCTCTGACCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA478200.18828641174529218Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCGATATCGTTCAGCGGTTAGGATATGTGAGATCGGAAGAGCACACGTCT404910.15942921576701435Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAAGTGGGCTCTGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC400490.15768888548697632Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTGGGCTCTGACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA382450.15058581800917398TruSeq Adapter, Index 2 (100% over 36bp)
AAGTCTCTAAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG371130.14612868254084127TruSeq Adapter, Index 2 (100% over 38bp)
AGTGGGCTCTGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA368410.14505771006081786TruSeq Adapter, Index 2 (100% over 36bp)
CCGACCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATC362890.1428842659101821TruSeq Adapter, Index 2 (100% over 42bp)
ATTGACTGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA353380.13913979962892378TruSeq Adapter, Index 2 (100% over 40bp)
GGTGCGTGAGGATTGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC350250.13790739379713216Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGGACTGCGAGCCTGAGAGGGTGTAGATCGGAAGAGCACACGTCTGAACTC347090.13666317576886963Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTGGGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG344450.13562370247943517TruSeq Adapter, Index 2 (100% over 35bp)
TCCGATATCGTTCAGCGGTTAGGATATGAGATCGGAAGAGCACACGTCTGA344010.13545045693119606Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGTGGGCTCTGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC331170.13039483684167383Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCACCGCTGGTCTAATGGTAGAATTTCACTAGATCGGAAGAGCACACGTCT316510.12462261016625352Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTTTGGTACTGTGGATAGGGGTAGATCGGAAGAGCACACGTCTGAACTCC296970.11692893286490887Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACAAGTGGGCTCTGACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC296710.11682656049549486Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGGAGAGTCGGATACTTTGAGAGTAGATCGGAAGAGCACACGTCTGAAC294950.11613357830253855Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGTGAACGAAGGTACTGTAAGCAGTAGATCGGAAGAGCACACGTCTGAA291890.1149287342625122Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTGGGCTCTGGGGGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC291220.11466492854132998Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGTGGGCTCTGACCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC286100.11264898034363885Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGAAATGTAGTCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC272810.10741617730705387Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCAGCAACTCTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA264540.10415994847992388TruSeq Adapter, Index 2 (100% over 36bp)
CAATAGGACTGCGAGCCTGAGAGGGTGCAGATCGGAAGAGCACACGTCTGA263900.10390795495521248Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGGTCTAATGGTAGAATTTCACCAGATCGGAAGAGCACACGTCTGAACTC260290.10248655397988729Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGGCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT259740.10226999704458845TruSeq Adapter, Index 2 (100% over 41bp)
AGAAGACATTGTGTGAACGAAGGTAGATCGGAAGAGCACACGTCTGAACTC259240.10207312710340767Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCAGCAGCAACTCTGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC255240.10049816757396147Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT254260.10011230248924716TruSeq Adapter, Index 2 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)