FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23474521
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4057961.7286657308151252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA16100.013.42401750-51
GCCGTAT18350.011.518990548-49
CGCCGTA18350.011.38901846-47
CCGGGAT14850.011.2211181
CGCGTTA3550.001681233410.7033968
CCGTATC20150.010.60786448-49
GCGTTAG4054.2366082E-410.5548069
CGGGATT8752.3101165E-1010.32650852
CGGGGAT17150.010.2714591
TCGCCGT20600.09.79921746-47
CGGTTCG6101.8189894E-129.3434312-13
CACTCTT35700.08.791922
GCGGGAT10556.684786E-98.5742411
CGGGGTT21700.08.3371641
CGCGGGT12008.949428E-108.3316791
CTCGGTT10901.1976226E-88.2989221
CTCGGGT13701.7462298E-107.99285271
CGGGTCT20350.07.9410853
CCCGTTT10951.13692295E-77.8262361
GTATCAT28050.07.620380450-51
Produced by FastQC (version 0.11.5)