FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n02_ALm216.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n02_ALm216.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14560738
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT31000.012.1268791
TACCGTA14400.011.5445539
CTACCGT19050.09.7237828
CGGGGAT48550.08.2332951
GGGGAAT79250.08.2263081
CGGGGTT48350.08.1689331
CGAATCG8802.6322323E-68.1006873
GCGTCGT125550.08.039700594-95
CCGGGAT40950.08.0182361
CGACCGT11302.2933818E-88.0012671
CGCATAT9851.5421065E-67.7297851
CGGGATT25550.07.6361881
AGGGGGG134350.07.601276482-83
AGCGTCG144200.07.54341394-95
CGTATCG17651.8189894E-127.53922273
CGTCGTG123150.07.521385794-95
CGCGAAT9507.6617725E-67.5136561
CGGGAAT33900.07.43977551
TAGGGGG46150.07.41072550-51
ATCGGAA278050.07.371495294-95
Produced by FastQC (version 0.11.5)