FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n02_ALm215.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n02_ALm215.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16842552
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT36000.09.914981
TACCGTA16000.09.2026999
AAGGGGG144050.09.00193380-81
CGGGGAT55550.08.4817261
CGGGAAT35350.08.3470951
GTCCGAT12854.0017767E-108.148031
CCGGGAT49150.08.0368831
GCGTCGT139050.08.02790194-95
CGGGATT27350.08.0044881
CGGGGTT53350.07.85021071
TCGGATT26650.07.84986932
AAAAGGG177500.07.840574778-79
ATCGGAA311550.07.829207494-95
GATCGGA332300.07.797753394-95
AGCGTCG160500.07.75409994-95
AGGGGGG163650.07.619086382-83
AGATCGG375950.07.580995694-95
AAAGGGG156650.07.565306780-81
CCCGTAT26450.07.5571271
GTCGGAT19550.07.5465421
Produced by FastQC (version 0.11.5)