FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n02_ALm214.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n02_ALm214.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16439773
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA15550.011.3017779
CGTATCG19600.010.1845573
CGGGGAT55850.09.7150641
CGCGGGT33050.09.5046571
AGGGGGG147150.08.84439982-83
CTACCGT21350.08.6764248
GGGGGGA136550.08.601622
TAGGGGG54300.08.5290550-51
CCCGTAT25250.08.2938321
AAAGGGG151350.08.26932980-81
GTATCGA24500.08.14424
CGGGATT29300.08.1220641
CGGGGTT52800.08.0226911
AAGGGGG139000.07.82515280-81
TCGGATA18050.07.6400432
GATCGGA320550.07.594489694-95
CTCGACT27600.07.5876551
GGGGAAT93150.07.4088211
AGATCGG360400.07.34785594-95
CGACCGT15101.36788E-97.24961421
Produced by FastQC (version 0.11.5)