FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n02_ALm213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n02_ALm213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16425795
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA16850.011.5574149
CGCGGGT33050.010.8009961
CTACCGT22650.09.2269018
CGGGGAT54650.09.0576841
CCGGAAT37650.08.5964261
CGCGAAT10052.676461E-88.5247261
GGGGAAT88050.08.2705821
GTCGAAA25900.08.2696431
CGATCGT5250.0045119748.143784
CGCGAGT17000.08.1193841
CGACCGT14701.0913936E-118.0946241
CGGGAAT41650.07.9993931
CGCGTGT22650.07.9852661
CGGGGTT55650.07.9541131
AAGGGGG140650.07.85166780-81
CGCGGAT21250.07.8394051
GCGTCGT148500.07.77275694-95
CGGGTCT60350.07.71757273
CCGGGAT45750.07.6986511
GATCGGA340350.07.66199494-95
Produced by FastQC (version 0.11.5)