FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n02_ALm212.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n02_ALm212.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17607334
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT36750.09.8423981
TACCGTA16150.09.4113469
AAGGGGG160250.08.62537380-81
CGGGGTT59000.08.5506381
CGCTAAT8006.2928666E-68.3288051
GTCGATT14509.094947E-128.205721
CGGGAAT37200.08.1880731
CCGGGAT53600.08.1689781
GCGTCGT146000.08.16631294-95
AGGGGGG175900.08.1280982-83
CGGGTAT18250.07.82353541
CTACCGT19450.07.81454048
AAAGGGG170950.07.752075780-81
CGCGTAA6150.00211671097.73872761
GGGAAAT143200.07.71062661
CGGGGAT63400.07.6569461
GTCGAAA28650.07.64148661
AAAAGGG189000.07.577097478-79
TCGGATT28550.07.49453452
TCGTGTA125300.07.430364694-95
Produced by FastQC (version 0.11.5)