FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n02_ALm211.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n02_ALm211.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17481794
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT3206.9367996E-411.8980681
CGCGGGT37700.010.3516341
AGGGGGG164150.08.95576382-83
CGGGAAT38800.08.8315551
CCGGGAT51200.08.64469051
AAGGGGG158550.08.43314180-81
GGGGGGA152150.08.4066392
GTCCGTT17000.08.3986361
CGGCATT31900.08.20556451
AAAGGGG174900.08.06573480-81
CGGGGAT56250.08.0378061
CTCGGTT33900.07.86185071
GGGGAAT98350.07.8392971
GTCGGAT18350.07.78075271
GGCGAAT23450.07.71218061
GATCGGA345650.07.407108394-95
TAGGGGG57800.07.35502650-51
AAAAGGG187950.07.202379778-79
ATCGGAA325550.07.17866894-95
CGGGGTT58350.07.1775831