FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n02_ALm210.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n02_ALm210.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17904760
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA19250.013.817429
CTACCGT25800.011.4140678
CGGGGAT50200.010.6192371
AAGGGGG163300.010.06391280-81
CGACCGT13300.010.0204081
CGCGGGT30700.09.6124111
AGGGGGG175100.09.38574382-83
TAGGGGG56850.09.19075750-51
AAAGGGG179050.08.78072980-81
AAAAGGG203200.08.46169978-79
CCGGGAT47950.08.3381491
CGGGAAT43400.08.3349481
CGCGGTT20000.08.3294641
CGCGAAT9356.996743E-78.1449311
ACTACCG32050.08.0027237
CGCGGAT22700.07.96776961
CGAGAAT38850.07.9634511
CGTATGG17650.07.93858912-13
CTCGGTT31250.07.92013031
CTCGGAT49700.07.565711
Produced by FastQC (version 0.11.5)