FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n02_ALm209.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n02_ALm209.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18286170
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA17750.010.1687559
TAGGGGG59950.09.74558650-51
AGGGGGG185600.09.66098882-83
AAGGGGG169850.09.57799480-81
AAAGGGG186900.09.33958480-81
CGCGGGT33850.09.1389321
CGCGATT6302.9607489E-59.0652871
GGGGGGA159400.08.441492
CGGGGAT56050.08.4062131
GCGTCGT172050.08.26861994-95
AAAAGGG206650.08.11364478-79
CGCGAAT10052.4262954E-78.0505151
CGCGAGT17900.07.9764391
GATCGGA393400.07.55841794-95
GGGGGGT182450.07.52364314-15
CCGGAAT41550.07.3307721
ATCGGAA371550.07.31256394-95
AGCGTCG195250.07.29828994-95
AGATCGG442000.07.291526394-95
CGGGTAT20350.07.25000141
Produced by FastQC (version 0.11.5)