FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n02_ALm208.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n02_ALm208.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18340884
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT35250.012.0163311
TACCGTA19450.010.2567049
GGGGAAT94500.08.7631131
CGGGGAT55750.08.7075571
CGACCTA10052.4975998E-78.0359634
GCGTCGT166300.07.940836494-95
AGCGTCG188450.07.92753594-95
CGACCGT16803.6379788E-127.6488381
GATCGGA396450.07.626471594-95
CTACCGT23800.07.5837228
AAGGGGG156200.07.49581480-81
CCCGTAT31200.07.4745061
AGAGCGT231800.07.459360694-95
ATCGGAA374650.07.391906794-95
CGCGGTT24600.07.3517291
CGGGATT33400.07.267161
CCCGATT28200.07.0882851
AGATCGG441100.07.08602294-95
GAGCGTC228100.07.04931794-95
CGTCGTG163750.06.96215494-95
Produced by FastQC (version 0.11.5)