FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n02_ALm207.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n02_ALm207.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19453059
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT37550.010.7744811
TACCGTA20000.010.4496459
CGGGGTT64300.09.5491811
CGGGAAT47250.08.9655221
CGTATCG22900.08.9246913
CGCGTAA4300.0080529688.8554151
CGGGGAT60600.08.1686091
GCGTCGT187450.08.15977894-95
CGCCAAT35050.08.1479921
CTACCGT27400.07.974128
CTCGTAT21000.07.9329761
AGCGTCG217350.07.791260794-95
GGGGAAT97900.07.5845411
AGAGCGT258050.07.43678794-95
ATCGGAA417700.07.346422794-95
GATCGGA445600.07.25955294-95
GAGCGTC258500.07.258458694-95
CGGGATT33450.07.2570721
AGATCGG502000.07.252977494-95
CGTCGTG181100.07.213102394-95
Produced by FastQC (version 0.11.5)