FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n02_ALm206.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n02_ALm206.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18398863
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT4458.336257E-510.6954261
TACCGTA16750.010.4919579
CGCGGGT37700.09.72092151
CGGGGAT58150.09.1669831
CGGTAAT19300.08.8777581
CCCGTAT28100.08.6381731
CGCGAGT18400.08.53599551
GCGTCGT196150.08.39107494-95
CGGGGTT61750.08.1700931
TAGGGGG56700.08.16797150-51
GGGGAAT101600.08.15105151
AGCGTCG224400.07.99091994-95
CGCGGAT25000.07.8055221
AGGGGGG163800.07.771379582-83
CGGGTAT20250.07.52112871
CGTCGTG190200.07.39237294-95
GGGGGGA150250.07.37394572
ATCGGAA432850.07.36904594-95
GATCGGA461850.07.364015694-95
CGCGTTT14951.1095835E-97.3222531