FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n02_ALm205.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n02_ALm205.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17984529
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA16300.014.8610889
CTACCGT23150.010.6689188
CGGGGAT57450.09.6929491
CGCGGGT36650.09.4800141
CGGGTAT17850.08.799051
GCGTCGT169550.08.48891494-95
GTCGGAT18100.08.4145611
AAGGGGG163550.08.2914880-81
ACCGTAT18100.08.1346210-11
ACTACCG30750.08.0321377
GGGGAAT99550.07.98427061
CGCTAAT7804.125951E-57.93247651
AGGGGGG173350.07.91873782-83
GGGGTAT54750.07.9107441
AGCGTCG193650.07.8249394-95
TCGGATT30750.07.7318722
CCGGGAT50050.07.70266441
CGTCGTG166350.07.66705894-95
CGGGGTT63400.07.65721751
GATCGGA397150.07.56507594-95
Produced by FastQC (version 0.11.5)