FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n02_ALm204.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n02_ALm204.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18680102
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA19200.010.6374269
CGCGAAT10950.010.4333621
CGCGGGT37500.09.6473821
CTACCGT23600.09.2579088
CGGGGAT58400.09.1291921
CGGGATT34650.08.3801871
GGGGAAT96800.08.3598931
AAGGGGG162200.07.87720180-81
CCGGGAT54500.07.8609161
CTCGGGT50200.07.775661
GCGTCGT164750.07.697803594-95
CGGGAAT44650.07.6760571
ACTACCG29450.07.58022177
GATCGGA370950.07.413856594-95
AGGGGGG170300.07.293407482-83
AGCGTCG189100.07.24661694-95
GGGAAAT151400.07.16862961
AGATCGG416100.07.16303494-95
ATCGGAA350450.07.149527594-95
CTCGGTT34650.07.1437661
Produced by FastQC (version 0.11.5)