FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n02_ALm203.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n02_ALm203.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17827201
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT4152.9653092E-612.6132111
TACCGTA18400.010.0673369
CGGGGAT58250.09.2313151
CGCGATT4700.0016306199.1122521
CGGGGTT62200.08.87459851
CTACCGT22650.08.5976268
CGCGAAT10657.9835445E-98.4895571
CGGGAAT44550.08.4384061
AAGGGGG158200.08.36175680-81
GCGGGTT30900.08.3160351
CGCGGGT36700.08.1687481
AGGGGGG162250.07.90434282-83
CCGGGAT54000.07.84291121
GCGTCGT143100.07.75081494-95
CGGGTAT18050.07.64542871
GTCGATT15051.6189006E-107.58849761
CTCGGAT48600.07.5393781
AGCGTCG163150.07.51160494-95
AGATCGG356600.07.446129394-95
CTCGGTT36300.07.3411221
Produced by FastQC (version 0.11.5)