FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n02_ALm202.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n02_ALm202.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17719859
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA17350.012.5932269
GTCCGAT16200.010.8694641
CTACCGT22300.09.7977588
CGGGAAT40050.09.2685851
CGGGTAT20050.09.019671
CGCGGGT38050.08.7551741
CGGGGAT55300.08.60591
CGGGGTT60950.08.5889561
AAGGGGG149650.08.49042480-81
GGGGAAT92450.08.3907751
CGATTGG16800.08.2003714
ACTACCG30200.08.1784797
GTCGAAT16201.8189894E-127.9317711
CCGGGAT51550.07.8471441
CCCGTAT26900.07.784341
AAAAGGG182550.07.545638678-79
CGCGGAT21250.07.3905441
GCGTCGT161650.07.34612494-95
TCGAATC20750.07.3325182
AGCGTCG186550.07.180386594-95
Produced by FastQC (version 0.11.5)