FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n02_ALm201.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n02_ALm201.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17899310
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA17350.09.3079959
CTCGGAT48500.09.2255171
CGGGGAT57850.08.5572571
CGCGGGT35150.08.3959711
AGGGGGG160100.08.14403982-83
CGGGGTT59300.08.02693751
AAGGGGG156000.07.825190580-81
GGGGAAT98750.07.76056581
CGCGGAT20900.07.7434991
CTACCGT23400.07.71335368
GATCGGA314950.07.67668194-95
TAGGGGG55400.07.67367450-51
AAAGGGG169300.07.39281980-81
CGACCTA9659.775253E-67.38515474
GGGGGGA142100.07.1944392
AGATCGG352350.07.16516794-95
ATCGGAA298050.07.163708794-95
GGGAAAT142300.07.09145831
CGGGATT27600.07.07097631
AGCGTCG159200.07.02659994-95
Produced by FastQC (version 0.11.5)