FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23474521
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4032191.7176878710325976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG11300.023.33755134-35
TATCTCG15050.018.1533940-41
AATGCGC16400.017.23907334-35
AGAGCAC106600.015.4576118
TCTCGTA36850.014.95531542-43
ACGTCTG52500.014.4720914-15
CGTCTGA56500.013.95206716-17
CTCGTAT34600.013.6617544-45
ACACGTC56700.013.5675712-13
TGCGCAT23300.013.56121536-37
CACGTCT58350.013.30599814-15
ATGCCGT45000.013.08961448-49
GCTATCT23200.012.90266638-39
TTCCCTA25350.011.9887018
CGCATCT24800.011.78284938-39
ATCTCGT40400.011.75963142-43
CACACGT67150.011.63295412-13
ATGCGCA23100.011.62169236-37
CTAATGC24250.011.56076532-33
GTCACTA26000.011.51317228-29
Produced by FastQC (version 0.11.5)