FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n01_ALm216.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n01_ALm216.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14560738
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT380780.2615114700916945TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG56300.034.08653648-49
TCGTATG54900.033.2287744-45
CGTATGC57450.033.0321346-47
TAATGCG59450.032.76907734-35
CTCGTAT57750.032.28814344-45
AATGCGC71200.026.82738334-35
TCTCGTA68650.026.47007842-43
ATCTCGT75800.024.53730842-43
TGCGCAT84550.022.78746836-37
GTATGCC83550.022.77015546-47
ATGCCGT85700.022.22662248-49
ATGCGCA89000.021.9684136-37
GCGCATC88150.021.7756538-39
CTAATGC90700.021.2699632-33
CCGTCTT90950.020.6816552-53
GCCGTCT89900.020.47420350-51
CGCATCT93900.020.01211438-39
ACTAATG103750.019.32734532-33
GTCACTA103350.018.7117328-29
TGCCGTC103150.018.67310750-51
Produced by FastQC (version 0.11.5)