FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n01_ALm215.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n01_ALm215.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16842552
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT421520.25027086156539696TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC63400.036.03890646-47
TCGTATG61850.035.71719744-45
TATGCCG64450.035.5253248-49
TAATGCG68450.034.32744634-35
CTCGTAT66600.033.20546744-45
TCTCGTA75100.029.19541442-43
AATGCGC81750.028.568334-35
ATCTCGT84250.026.53215842-43
TGCGCAT97250.023.8438136-37
ATGCGCA101300.023.42995636-37
GCGCATC100150.023.22447438-39
ATGCCGT97750.023.18010948-49
GTATGCC100550.022.81817246-47
CTAATGC103100.022.65212832-33
GCCGTCT104300.021.33642850-51
CCGTCTT106700.021.25716652-53
CGCATCT107750.020.79258738-39
CATCTCG110300.020.18244240-41
GTCACTA120100.019.62314628-29
ACTAATG124250.019.40813432-33
Produced by FastQC (version 0.11.5)