FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n01_ALm214.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n01_ALm214.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16439773
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT430850.26207782796027657TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG63550.035.35895544-45
CGTATGC64550.035.21280346-47
TATGCCG65250.034.94418748-49
TAATGCG69350.033.63971334-35
CTCGTAT67200.033.22633444-45
AATGCGC81900.028.3978934-35
TCTCGTA80050.027.59679442-43
ATCTCGT88400.025.09756942-43
TGCGCAT95000.024.33133536-37
GCGCATC96400.023.60854138-39
ATGCGCA99450.023.57702836-37
ATGCCGT98250.023.3280848-49
GTATGCC100650.022.84264646-47
CCGTCTT104800.021.8917752-53
CTAATGC106600.021.8630632-33
CGCATCT102850.021.82771138-39
GCCGTCT104950.021.58892350-51
CATCTCG109750.020.43362840-41
GTCACTA117600.019.93871128-29
ACTAATG118100.019.93530732-33
Produced by FastQC (version 0.11.5)