FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n01_ALm213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n01_ALm213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16425795
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT399910.24346462378228875TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC62700.034.886846-47
TATGCCG64000.034.43824448-49
TCGTATG61950.034.3545344-45
TAATGCG67350.033.19382534-35
CTCGTAT65400.032.72384644-45
AATGCGC79350.027.66497634-35
TCTCGTA75750.027.56436742-43
ATCTCGT85000.025.2633742-43
GTATGCC92450.023.50624546-47
GCGCATC93300.023.50278738-39
ATGCCGT95250.023.11467648-49
ATGCGCA97850.023.065636-37
TGCGCAT94200.023.02617636-37
GCCGTCT98750.022.0062750-51
CTAATGC106400.020.94439532-33
CCGTCTT105850.020.82205252-53
CGCATCT104300.020.38628238-39
CATCTCG106200.020.06593140-41
GTCACTA116800.019.11963828-29
TGCCGTC116800.019.11381750-51
Produced by FastQC (version 0.11.5)