FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n01_ALm212.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n01_ALm212.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17607334
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT484400.27511263204298847TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG69900.036.87172744-45
CGTATGC72900.036.4905846-47
TATGCCG73300.036.3884648-49
TAATGCG77300.034.8837134-35
CTCGTAT74250.034.8395344-45
AATGCGC89400.029.79026634-35
TCTCGTA87000.029.21608742-43
ATCTCGT96250.027.02532642-43
TGCGCAT103600.025.82159636-37
ATGCCGT103150.025.58189448-49
ATGCGCA107800.025.23429536-37
GTATGCC107200.024.9257446-47
GCGCATC106900.024.73574338-39
CTAATGC115850.023.13203232-33
GCCGTCT116900.022.20639650-51
CGCATCT117100.022.15507738-39
CCGTCTT120650.021.75234852-53
TGCCGTC126850.021.41943250-51
CATCTCG121150.021.11983940-41
ACTAATG134700.020.3005832-33
Produced by FastQC (version 0.11.5)