FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n01_ALm211.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n01_ALm211.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17481794
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT472820.2704642326754336TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC73250.035.47229846-47
TCGTATG71350.035.3224444-45
TATGCCG74950.034.9843648-49
CTCGTAT76050.033.6704444-45
TAATGCG81250.032.89462734-35
TCTCGTA88050.028.54322242-43
AATGCGC92650.028.48816334-35
ATCTCGT97300.026.17150342-43
GCGCATC110000.024.2323438-39
TGCGCAT109200.024.1922436-37
ATGCCGT106900.024.03953648-49
ATGCGCA111750.024.00160836-37
GTATGCC112550.023.17055546-47
CCGTCTT115800.022.64267252-53
GCCGTCT114900.022.22046550-51
CGCATCT116850.021.85621338-39
CTAATGC122050.021.70387332-33
CATCTCG121600.020.84594740-41
GTCACTA131600.020.5073828-29
ACTAATG133050.020.4451732-33
Produced by FastQC (version 0.11.5)