FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n01_ALm210.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n01_ALm210.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17904760
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT582820.32551120484161755TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG82400.037.38770744-45
TATGCCG85400.036.9332448-49
CGTATGC85650.036.68607346-47
TAATGCG94500.034.5172934-35
CTCGTAT91300.034.2374344-45
AATGCGC105650.030.3797234-35
TCTCGTA103550.029.79842442-43
ATCTCGT108100.028.80787742-43
TGCGCAT119000.026.73223136-37
GCGCATC119450.026.6711938-39
ATGCCGT117050.026.62193748-49
ATGCGCA123550.026.26694136-37
CCGTCTT122800.025.58720852-53
GTATGCC124350.025.23049546-47
GCCGTCT122350.025.17651450-51
CTAATGC135950.023.85352732-33
CGCATCT132000.023.81144138-39
CATCTCG133600.023.0457940-41
TGCCGTC141950.022.118550-51
ACTAATG153150.021.53136832-33
Produced by FastQC (version 0.11.5)