FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n01_ALm209.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n01_ALm209.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18286170
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT625310.34195788401835925TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC86400.038.0170846-47
TCGTATG84750.037.78063244-45
TATGCCG87800.037.54588348-49
CTCGTAT90150.035.8864444-45
TAATGCG97550.034.7757934-35
TCTCGTA101600.031.44531242-43
AATGCGC109800.030.63634934-35
ATCTCGT112450.028.91820542-43
ATGCGCA129100.026.55326736-37
TGCGCAT126700.026.54999736-37
GTATGCC127900.025.90442846-47
GCGCATC130450.025.67743738-39
ATGCCGT128300.025.45325948-49
CCGTCTT130300.025.42670252-53
GCCGTCT130250.024.90753750-51
CTAATGC140050.024.2397532-33
CGCATCT135950.024.0619838-39
CATCTCG138750.023.26789940-41
GTCACTA147900.022.82422428-29
ACTAATG155450.022.12875232-33
Produced by FastQC (version 0.11.5)