FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n01_ALm208.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n01_ALm208.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18340884
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT390010.21264514840178914TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG61900.033.7312544-45
CGTATGC64500.033.21513746-47
TATGCCG65550.032.64583248-49
CTCGTAT66700.031.76678544-45
TATCTCG75050.027.41337240-41
TCTCGTA80750.025.71122242-43
ATCTCGT93200.022.65894342-43
GCTATCT97750.021.12047238-39
GTATGCC104850.020.16094246-47
CCGTCTT113150.019.54125252-53
ATGCCGT108600.019.42042748-49
GCCGTCT110800.019.39830250-51
AGCTATC112400.018.93835638-39
CTATCTC119900.018.05071640-41
TGCCGTC134300.016.44605850-51
AAGCTAT136250.015.95459636-37
CGTCTTC138600.015.76456152-53
GAAGCTA141800.015.6484736-37
AGGGGGG138350.013.8527274-75
AGTCACC178450.012.56775928-29
Produced by FastQC (version 0.11.5)