FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n01_ALm207.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n01_ALm207.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19453059
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT386080.19846750066403437TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG61200.032.75747344-45
CGTATGC64100.031.86565846-47
TATGCCG67000.031.0537948-49
CTCGTAT67350.030.22473744-45
TATCTCG73400.027.12313740-41
TCTCGTA81050.024.67738342-43
ATCTCGT92450.022.25107642-43
GCTATCT99650.020.09771338-39
ATGCCGT104250.019.59330448-49
GTATGCC105350.019.45623246-47
CCGTCTT112100.018.7502652-53
GCCGTCT110600.018.68249550-51
AGCTATC113650.017.99824138-39
CTATCTC122250.017.062340-41
TGCCGTC131700.016.08606750-51
CGTCTTC136900.015.31887652-53
AAGCTAT140150.014.96802636-37
GAAGCTA146250.014.53865136-37
AGGGGGG141100.013.48181474-75
AGTCACC185350.011.586719528-29
Produced by FastQC (version 0.11.5)