FastQCFastQC Report
Thu 16 May 2019
HFHN2BGXB_n01_ALm206.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHN2BGXB_n01_ALm206.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18398863
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT407360.22140498573199877TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG63400.034.9187844-45
TATGCCG66300.033.74579248-49
CGTATGC66350.033.64808746-47
CTCGTAT68650.031.97156344-45
TATCTCG76700.028.371840-41
TCTCGTA83650.026.09733842-43
ATCTCGT92550.023.69037242-43
GCTATCT99800.021.9241338-39
ATGCCGT103300.021.79667948-49
GTATGCC105100.021.49073246-47
GCCGTCT109700.020.67617450-51
CCGTCTT111300.020.40011452-53
AGCTATC115600.019.00978338-39
CTATCTC117200.018.87160540-41
TGCCGTC129600.017.6846250-51
AAGCTAT137850.016.510336-37
CGTCTTC141200.016.41666852-53
GAAGCTA149400.015.47242736-37
AGGGGGG142250.014.4581674-75
GCTTGAA183300.012.9584860-61
Produced by FastQC (version 0.11.5)